Detection of Haplosporidium pinnae from Pinna nobilis Faeces

Pinna nobilis (Linnaeus, 1758) is the largest bivalve endemic to the Mediterranean. It is distributed in a wide range of coastal environments, including estuaries. Pinna nobilis has recently become a critically endangered species (with almost 100% mortality) along the entire Spanish Mediterranean coast. This may be due to coinfections caused by Haplosporidium pinnae and bacterial pathogens such as Mycobacterium spp. We extensively sampled P. nobilis from Mar Menor lagoon (SE Spain), a site where individuals still survive. Using conventional PCR, we found Haplosporidium spp. in 7.1% of mantle and faecal DNA samples in different individuals of P. nobilis. We identified and quantified Haplosporidium pinnae in P. nobilis using Sanger sequencing and qPCR. Faecal H. pinnae detection is non-invasive, unlike biopsies. Therefore, this non-lethal and non-invasive sampling method could contribute to the welfare of living populations, particularly in eutrophic environments, where they are prone to septicaemia. The use of faecal DNA analysis could be a major advance in epidemiology and recovery assessment studies of P. nobilis.

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Lopez-Nunez R. Cortes Melendreras E. Gimenez Casalduero F. Prado P. Lopez-Moya F. y Lopez-Llorca L.V. Detection of Haplosporidium pinnae from Pinna nobilis Faeces. MDPI, 2022. https://doi.org/10.3390/jmse10020276

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Tipo de recurso Artículo
Fecha de creación 05-11-2024
Fecha de última modificación 19-01-2025
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Identificador de los metadatos c70fc739-2f90-59f5-8b6a-13c64b266df4
Idioma de los metadatos Español
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Categoría del conjunto de alto valor (HVD) Observación de la Tierra y medio ambiente
Categoría temática ISO 19115 biota
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Nombre del autor Lopez-Nunez, R., Cortes Melendreras, E., Gimenez Casalduero, F., Prado, P., Lopez-Moya, F. y Lopez-Llorca, L.V.
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Identificador alternativo DOI: 10.3390/jmse10020276
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Email del autor rln3@gcloud.ua.es
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